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Basics of Extracellular Vesicles
  • Level Professional
  • Duration 10 hours
  • Course by University of California, Irvine
  • Offered by

About

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea).

Modules

Course Overview

    • 2 Videos
    • 1 Readings
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2 Videos

  • Introduction to the Field of Extracellular Vesicles
  • Introduction to the Course

1 Readings

  • Syllabus

Introduction to Extracellular Vesicles

    • 2 Videos
    • 1 Assignment
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1 Assignment

  • Introduction to Extracellular Vesicles

2 Videos

  • The Origin of EVs throughout the Phylogenetic Tree
  • History of Exosomes and Extracellular Vesicles

Biogenesis, Cargo and Uptake of Extracellular Vesicles - Part I

    • 3 Videos
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3 Videos

  • Biogenesis and Release of Extracellular Vesicles
  • Mechanisms of Extracellular Vesicle Uptake - Part 1
  • Mechanisms of Extracellular Vesicle Uptake - Part 2

Biogenesis, Cargo and Uptake of Extracellular Vesicles - Part II

    • 3 Videos
    • 1 Assignment
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1 Assignment

  • Biogenesis, Cargo and Uptake of Extracellular Vesicles

3 Videos

  • The Protein Content of Extracellular Vesicles
  • The RNA Content of Extracellular Vesicles
  • The Lipid Content of Extracellular Vesicles

Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles

    • 5 Videos
    • 1 Assignment
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1 Assignment

  • Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles

5 Videos

  • Cell Culture Media
  • Blood Plasma and Serum
  • Breast Milk
  • Urine
  • Cerebrospinal Fluid

Methods for Isolating Extracellular Vesicles - Part I

    • 4 Videos
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4 Videos

  • Differential Ultracentrifugation - Part 1
  • Differential Ultracentrifugation - Part 2
  • Density Gradient
  • Size Exclusion Chromatography

Methods for Isolating Extracellular Vesicles - Part II

    • 2 Videos
    • 1 Assignment
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1 Assignment

  • Methods for Isolating Extracellular Vesicles

2 Videos

  • Kit-based Precipitation
  • Summary of Isolation Methods for Extracellular Vesicles

Techniques for Characterization and Quantification of Extracellular Vesicles - Part I

    • 4 Videos
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4 Videos

  • Electron Microscopy - Part 1
  • Electron Microscopy - Part 2
  • Electron Microscopy - Part 3
  • Cryo-TEM

Techniques for Characterization and Quantification of Extracellular Vesicles - Part II

    • 3 Videos
    • 1 Assignment
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1 Assignment

  • Techniques for Characterization and Quantification of Extracellular Vesicles

3 Videos

  • AFM
  • Flow Cytometry
  • Nanoparticle Tracking Analysis

Auto Summary

Explore the fascinating world of extracellular vesicles (EVs) with this comprehensive course designed for science and engineering enthusiasts. Led by experts from top institutions like the International Society for Extracellular Vesicles (ISEV), University of California Irvine, University of Gothenburg, and Pohang University, this course delves into EV history, nomenclature, biogenesis, cargo, isolation methods, and characterization techniques. With five detailed modules and multiple choice exams, learners will gain a thorough understanding of EVs' roles and applications. Ideal for professionals with a background in biology and biochemistry, the course offers a professional-level education over 600 minutes with a starter subscription on Coursera.

Cecilia Lasser
Instructor

Cecilia Lasser